This is a comprehensive list of all boss summoning items and how to craft them, and it's intended to be used as a reference. The list includes simple instructions on where to get each ingredient. Some bosses have linked strategy guides in order to help players prepare for the fight. For more detailed info on bosses, see the main Bosses of Terraria Guide.
Most summoning items need to be used at night, which starts at 7:30PM. Day begins at 4:30AM, when many bosses will despawn. The few exceptions are noted below. It is possible to summon more than one boss at once, but not the same boss.
Summoning Item: Slime Crown
Materials to Craft the Slime Crown:
Notes: Craft at Demon/Crimson Altar. May also spawn randomly or when you kill 150 slimes during the randomly-occurring Slime Rain event. May be used at any time of day.
Summoning Item: Suspicious Looking Eye
Materials to Craft the Suspicious Looking Eye:
Notes: Craft at Demon/Crimson Altar, use at night. May also spawn randomly once you reach 200 health/10 defense and a few NPCs live in your town. This eye may be found randomly in chests while exploring.
Summoning Item: Worm Food
Materials to Craft Worm Food:
Notes: Craft at Demon/Crimson Altar. Must be used in the Corruption. Also summoned when the player breaks 3 corrupt orbs using a hammer. Every 3 will summon Eater of Worlds again.
Summoning Item: Bloody Spine
Materials to Craft Bloody Spine:
Notes: Craft at Demon/Crimson Altar. Must be used in the Crimson. Also summoned when the player breaks 3 crimson hearts using a hammer. Every 3 will summon Brain of Cthulhu again.
Summoning Item: Abeemination
Materials to Craft Abeemination:
Notes: Craft by hand (PC) or at Demon/Crimson Altar (Console/Mobile). Use anywhere in the Jungle to summon Queen Bee.
Summoning Item: Clothier Voodoo Doll
Required to re-Summon Skeletron:
Notes: Must have defeated Skeletron previously to gain access to the Dungeon. Do this by talking to the Old Man at night outside the Dungeon and inquiring about his 'curse'. While you have the Clothier Voodoo Doll, you can kill the Clothier NPC and Skeletron will be summoned.
Summoning Item: Guide Voodoo Doll
Required to Summon Wall of Flesh:
Notes: Underworld - the Guide must presently be alive, and will be killed. The Wall of Flesh will spawn on the side of the map you're closest to. Repeated farming of him is possible, although you must wait for the Guide to respawn.
Summoning Item: Mechanical Worm
Materials to Craft Mechanical Worm:
Notes: Craft at Mythril/Orichalcum Anvil. Before the defeat of it may be dropped by any enemy randomly at a very low chance. May also spawn randomly until then.
Summoning Item: Mechanical Eye
Materials to Craft Mechanical Eye:
Notes: Craft at Mythril/Orichalcum Anvil. Before the defeat of it may be dropped by any enemy randomly at a very low chance. May also spawn randomly until then.
Summoning Item: Mechanical Skull
Materials to Craft Mechanical Skull:
Notes: Craft at Mythril/Orichalcum Anvil. Before the defeat of Skeletron Prime it may be dropped by any enemy randomly at a very low chance. May also spawn randomly until then.
Summoning Item: Plantera's Bulb
Notes: All three Mechanical Bosses must have been defeated. Plantera's Bulbs will regularly re-grow in the Jungle. Break it with a pickaxe to start the fight at any time. This allows access to the Jungle Temple, and Golem.
Summoning Item: Lihzahrd Power Cell
How to get the Power Cell:
Notes: Farming monsters in the Jungle Temple is a good way to get many of these. You use the Power Cell by right-clicking the Altar. You can relocate the Altar using a Picksaw or better Pickaxe. You can then summon Golem anywhere.
Summoning Item: Suspicious Looking Skull
How to Craft the Suspicious Skull:
Notes: Evidently you may need another world if you did not get Adamantite in your current one, at least until the next Console/Mobile Terraria patch. Ocram drops Souls of Blight that are used in Console/Mobile exclusive gear.
Summoning Item: Truffle Worm
How to get Truffle Worm:
Notes: Use this as bait while fishing in the Ocean Biome on either side of the map. Make sure it's the bait that comes first in your inventory (top-left to bottom right). Cast your fishing pole, and Fishron will be summoned. Good luck, and don't leave the Ocean biome!
Summoning Item: None
Notes: Lunatic Cultist begins spawning after Golem is defeated. He spawns each day if the Lunar Event is not active. Kill him to initiate the Lunar Events and collect Solar/Vortex/Nebula/Stardust Fragments. When all 4 pillars are defeated, Moon Lord will spawn on top of the player. Be prepared for a fight, the Cultist is a genuine boss and challenging to newcomers.
Summoning Item: Celestial Sigil
Materials to Craft:
Notes: Craft at Ancient Manipulator (100% dropped by Lunatic Cultist). The Moon Lord can be summoned at any time. At first, it's better to summon him by doing the event, as you need those Fragments.
Invasions have mini-bosses of sorts, so these are worth listing here despite them not being bosses in the strictest sense. Here are what you need to start the game's Invasion Events.
Summoning Item: Pirate Map
Notes: These can be dropped by any monster in the Ocean Biome during Hard Mode. 1% chance, so set up to farm! The Pirate Invasion is worth farming as the pirates may drop the discount card and lucky coin, both of which have an impact on you financially - making monsters drop more coins, while also reducing prices by 20%.
Summoning Item: None
Materials to Craft:
Notes: Head to the outer third of your world. If you build uptward toward space, it's very likely one will spawn. Hang in the area and move about until you meet a probe, which will scan you. Let it live (do don't have any summon minions out), and the Martian Madness will ensue!
Summoning Item: Pumpkin Moon Medallion
Materials to Craft Pumpkin Moon Medallion:
Notes: Craft at Mythril/Orichalcum Anvil. You have from 7:30PM (when it can be used) until 4:30AM.
Summoning Item: Naughty Present
Materials to Craft Naughty Present:
Notes: Craft at Orichalcum/Mythril Anvil. You have from 7:30PM (when it can be used) until 4:30AM.
Summoning Item: None
Notes: Kill the Lunatic Cultist after having defeated Golem on PC. The event can be restarted the following morning if Moon Lord kills you, as the Cultists will respawn. You can summon Moon Lord directly once you've saved up Solar/Nebula fragments etc. after having done this event a few times.
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